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This first batch of analyses was conducted for two distinct purposes.
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Reproducibility of serum analyses was good; the ∑PCB within-batch coefficient of variation (CV) was 3% and the between-batch CV was 20% over the 5 years of analysis, with similar performance for pesticides.
The factor will be validated by applying it to analyses of several different experimental samples and measuring how close the reproducibility of these analyses are, using different batches of URR, relative to the reproducibility of the same analysis using a single batch of URR.
Each batch of flour was analysed by the National Institute for Scientific and Industrial Research, Lusaka, for macronutrients by proximate analysis and for iron and zinc by flame atomic absorption spectroscopy.
Each batch of samples was analysed in conjunction with Biophen low molecular weight heparin (LMWH) control plasma and Biophen heparin control plasma (Hyphen Biomed) as standards.
For each batch of analyses, the OPD reagent must be freshly prepared.
Negative controls (no DNA) and sequencing-verified positive controls were included in each batch of analyses.
Multiple aliquots of a control pooled serum sample were analyzed during each batch of analyses (intra-assay CV < 4.5%).
Negative controls and DNA-sequenced positive controls representing each possible genotype were included in each batch of analyses.
Negative and positive controls were included with each batch of samples being analysed.
Two slides from each batch of printing were analysed for spot quality using Ribogreen (Molecular Probes).
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