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Reagent kits with identical batch numbers were used for each analysis.
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Recently, we discovered a significant increase in VEGF levels when ELISA kits with the 'new' batch number were used.
All samples were analysed in a single batch, meaning that kits with identical lot numbers were used and all assays were performed by the same individual.
The product number is used to identify the characteristics of the prosthesis and the batch number is used to identify the specific production details of prostheses.
The same kit batch-number was used for all assay plates used in the study and the participant samples were distributed randomly on the analysis plates.
To avoid any plate-to-plate variation of anti-CCP measurements, plates from the same batch (batch number 470094) were used; the inter-assay and intra-assay variabilities were less than 9%.
Batch numbers were retained in order to adjust for possible batch-specific effects.
Two batches of sera were used: batch 1 (numbered 1 20 in Figures 1A, 1B and 1C and Figure 2A) and, when these were exhausted, batch 2 (numbered above 20, Figure 1D and Figures 2B, 2C).
Bones from the same batch were used to avoid any inter-batch variation in mechanical properties.
Reagents of the same batch were used.
Aliquots from the same batch were used for all experiments.
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