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For both methylation and RNA sequencing (RNA-Seq) experiments, statistical analyses are conducted for each batch independently and also by pooling both batches together before pre-processing the data.
However, since the paired PP and PN samples for all subjects belonged to the same batch and were processed simultaneously, it was not necessary to adjust for this effect, and we instead normalized data from each batch independently (and calculated PP – PN differences within each batch).
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A fractional factorial experimental design was applied to perform 32 cheese batches independently inoculated at 103 cfu/ml of milk using four strains producing SEA, SEB, SEC or SED.
A third batch of independently inoculated plants provided the mRNA templates used for this experiment.
Each batch was independently coded by two researchers (BB and RP), then reconciled and merged; the entire research team then resolved existing discrepancies in injury designations, and updated the qualitative coding structure when necessary.
Three batches of independently isolated RNAs were used as technical replications.
Three batches of independently generated BDS PM2.5 were analyzed by GC/MS after extraction with DCM.
No activity was observed in repeated assays and using several batches of independently purified recombinant protein (an example of activity assay data is shown in Figure 8B).
The batch kinetic tests were independently conducted to determine biokinetic parameters used as an input in the model.
The batch kinetic test was independently conducted to determine biokinetic parameters used in the microalgal biofilm model simulation while initial thickness of microalgal biofilm were assumed.
The adsorption isotherm parameters, the surface diffusion coefficient and its dependence on surface concentration were determined independently in batch adsorption studies.
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