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Batch fermentations were performed in a 1.2-liter fermenter and microfermenter (300 ml), with mixing by a magnetic stirrer (500 rpm), and airlocks to maintain anaerobiosis.
The batch fermentations were performed in either a 1.4-L fermenter with a 1000 mL working volume (Infors AG, Switzerland) or in 250-mL shake flasks with a 50-mL working volume.
All anaerobic batch fermentations were performed in triplicate in 2-l fermenters (Braun-Biostat B2, stirred tank, Braun Biotech, Melsungen, Germany) with a working volume of 1.5 l.
To select strains that were able to ferment D-xylose efficiently, small tube semi-anaerobic batch fermentations were performed at 35°C in 50 mL YP medium containing 40 g/L D-xylose as a carbon source.
For evaluation of the fermentation performance in medium containing D-xylose and glucose, the batch fermentations were performed in complex medium in 300 mL shake flasks with a working volume of 200 mL at 35°C.
Batch fermentations were performed with 10% algae as feedstock.
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Self-cycling fermentation (SCF) is a process in which sequential batch fermentations are performed using a computerized feedback control scheme.
After validation of the model in flasks, assays of batch fermentation were performed in a 7 l Infors HT fermenter (Infors AG, Rittergasse 27, 4013 Bottmingen, Switzerland) with a working volume of 3.5 l under the optimized culture conditions.
Three different repeated-batch fermentations were performed to characterize characteristics of the respiration-deficient mutant.
Intermittent and constant fed-batch fermentations were performed in the PFB to optimize the fermentation results.
Finally, pH-stat fed-batch fermentations were performed in a lab-scale (2 L) bioreactor with three different feeding solutions (glucose, yeast extract, and casamino acid).
More suggestions(14)
batch cultures were performed
batch studies were performed
batch fermentations were started
batch jobs were performed
batch runs were performed
batch blasts were performed
batch experiments were performed
batch tests were performed
batch fermentations were carried
batch fermentations were integrated
batch fermentations were developed
batch fermentations were undertaken
batch fermentations were conducted
batch cultivations were performed
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