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The resulting strain (JX123-BTT) was able to ferment a mixture of cellobiose and xylose simultaneously in batch fermentation with a high ethanol yield (0.38 g/g) and productivity (2.00 g/L⋅h).
An enhanced soluble protein, ChBD-SUMO-Hep I (CSH-I), was expressed in 3 L batch fermentation with a high bioactivity of 2.5 × 103 IU/L.
Next, two strains were compared in anaerobic batch fermentation with a mixture of glucose, arabinose and xylose as a carbon source (Table 2).
Fungal cultivations were run as batch fermentation with a substrate loading of 30 g DM pre-treated wheat straw per L (gDM WS/L).
The resulted engineered strain produced 20.4 g/l mixed alcohols (4.4 g/l isopropanol, 14.1 g/l butanol, and 1.9 g/l ethanol) in batch fermentation, with a yield to glucose of 0.3 g/g.
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Batch fermentations with a stable biofilm reactor could be completed in approximately 12 h, with a 5-fold higher productivity compared with a planktonic cell reactor.
To produce 3-HP from glycerol, fed-batch fermentation with a two-step feeding strategy was designed to separate the cell growth from the 3-HP production stages.
Optimization of processes using the AOX1 promoter in P. pastoris is generally done in bioreactors by fed-batch fermentation with a controlled continuous addition of methanol for avoiding methanol toxification and carbon/energy starvation.
Similarly, by applying gas stripping intermittently in fed-batch fermentation with a butanol tolerant strain, 172 g/L ABE (containing 113.3 g/L butanol) were produced with an overall ABE productivity of 0.53 g · L-1 · h-1[ 10].
Using fed-batch fermentation with an optimized medium, the engineered pathway could produce 4 g/L β-carotene, which was stored in lipid droplets within engineered Y. lipolytica cells.
The maximum l-lactic acid concentration of 182.0 g/liter was obtained from 30-liter fed-batch fermentation with an average productivity of 3.03 g/liter/h and product optical purity of 99.4%.
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