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Upon cultivating this E. coli strain in pure minimal media, the acetate concentration did not exceed values of 0.35 g L−1, even when the batch fermentation was started with a glucose concentration of 130 g L−1.
Subsequently, the medium was circulated again, and the next batch fermentation was started with the biofilm on the fibrous matrix.
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The batch fermentations were started by growing cells on 30 g of glucose in an initial working volume of 300 mL.
The batch phase of the fermentation was started by addition of 125 ml preculture grown in minimal glycerol media to 1.25 l basal salt medium (BSM; Invitrogen) supplied with 5.5 ml PTM1 trace salts (Invitrogen) and adjusted to the final pH.
Once the glucose was exhausted, which occurred approximately at 12 h of the initial batch fermentation, the second phase of the fermentation was started with fed-batch mode.
The fermentation was started with an eight-hour batch phase, using 438 mL WL liquid (130.2 g/L glucose).
Batch fermentation was perform with 200 g/l glucose concentration.
Each batch fermentation was conducted in duplicate.
Batch fermentation was conducted in a bioreactor using these parameters.
Batch fermentation was performed in a 10 l bioreactor (B-Braun).
Anaerobic batch fermentation was performed in either 3 l Biostat Bio Reactors (B.
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