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The inoculum for batch fermentation was prepared by growing the mutants in MTC medium overnight at 55°C in an anaerobic chamber (COY Laboratory Products, Inc., Grass Lake, MI, USA).
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The mash for ethanol fermentation was prepared in batches of 1000 g total weight.
Batch fermentation was perform with 200 g/l glucose concentration.
Each batch fermentation was conducted in duplicate.
Batch fermentation was conducted in a bioreactor using these parameters.
Batch fermentation was performed in a 10 l bioreactor (B-Braun).
Anaerobic batch fermentation was performed in either 3 l Biostat Bio Reactors (B.
Batch fermentation was performed using sugarcane juice as the carbon source.
The pH of the batch fermentation was controlled at pH 5.8 and temperature 33°C.
Various fermentation processes such as wet fermentation, dry fermentation, and batch fermentation were analyzed according to the emission situation.
When batch fermentation is carried out without pH control, pH decreases during fermentation, affecting the ethanol production and the productivity.
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