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In batch fermentation, using the designed medium MW1 and MW2, the plasmid DNA yield was 60±5.0 and 30±4.0 mg/l, respectively.
In the scale-up experiment, seed inoculums were inoculated into a 10-L bioreactor (BioFlo 310, Eastbio, China) for batch fermentation using the optimum conditions determined in the shake-flask experiments.
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Pool experiments were performed as 1.2-liter batch fermentations using the homozygous deletant pool.
Moreover, FPase and xylanase titers of 2.63 and 108.72 IU/mL, respectively, were achieved using glucose as sole carbon source within 48 h in a 7-L fermenter by batch fermentation using T. reesei zxy-2.
The optimum conditions were applied in a batch fermentation using a 2-L fermenter with agitation rate of 100 rpm at 37ºC.
To evaluate more precisely the D-xylose and glucose utilization rates in the spruce hydrolysate, we studied the performance of the three strains in a similar batch fermentation using undetoxified spruce hydrolysate at a solid loading of 11% (corresponding to about 50% of the slurry), and supplemented with glucose and D-xylose to a final concentration of 62 g/L and 18 g/L, respectively.
The expression patterns of the glycolytic genes observed here are generally similar to those described earlier when the transcriptome of the same strain was examined during a batch fermentation using a different growth medium [ 9].
In batch fermentation using rapeseed meal as a nitrogen source, DO decreased to 0%% rapidly during the early stage (0 10 h) and then remained at 0%% until 42 h (Fig. 4a), indicating a high oxygen demand during this period.
The production of thermo-alkali-stable and cellulase-free xylanase of polyextremophilic novel Bacillus halodurans TSEV1 (MTCC 10962) was optimized in batch fermentation using statistical approaches.
Figure 7 R-3-HB production during batch fermentation using 50 g/L glucose or 50 g/L xylose as substrate (xylose: green diamond symbol; glucose: ■; xylose with extra nitrate and chloride ions red circle symbol; xylose with extra nitrate ion blue up pointing triangle symbol).
The solvent concentration and productivity increased by 4 to 6-fold and 3 to 5-fold, respectively, compared to traditional batch fermentation using planktonic culture.
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