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These batch effects were found to potentially cause spurious detection of bimodality, and hence the analyses were performed separately in two groups of identifiable chips of size 71 and 47.
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A suggestion of a batch effect was found by the y of interview, which corresponds to time of inclusion and blood collection and was not related to case/control status (Supplemental Material, Figure S2).
No other group effects were found.
No other significant effects were found.
No effects were found for the controls.
The following significant effects were found.
Possible batch artifacts were investigated and no significant effect was found, therefore staining batch was not considered as a factor for analysis.
No other effect was found.
Batch effects were removed by aligning the within-batch medians for all measurements.
Batch effects were then adjusted by the COMBAT method [ 30].
Experimental batch effects were adjusted for by including experimental batch as a covariate in the statistical model [ 56].
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