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Batch cultures were used to further isolate the pharmaceutical degrading bacteria by dilution series in either aerobic liquid medium or on agar plates.
Samples equivalent to 62.5 mg of dry weight biomass taken at the mid-exponential phase (ca. 10 h after inoculation) of aerobic batch cultures were used to obtain cell extracts as previously described (58).
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Batch culture was used to examine the effect of nitrate and NH4Cl on ruminal methane production and fermentation characters.
This growing batch culture was used as a starting point for further fed-batch induction, in which a media without glucose containing 1 mM IPTG and 0.4 M saccharose was continuously added.
To determine how the mutations whose wild-type expression had no effect were contributing to the oscillator, pools of spores from a diploid heterozygous for the eight mutations were selected for four 'sub-traits' of the oscillator: batch culture was used to select for exponential growth and FACS to select for constitutive clumps, single cells, and stochastic clumping.
Fed-batch cultures are used in producing monoclonal antibodies industrially.
The example of sugar uptake (phosphotransferase system, PTS) of E. coli cells growing in a fed-batch culture is used to illustrate the application of the approach.
Repeated fed-batch culture was used for the enrichment of heat-shock treated anaerobic sludge, in a 1 L serum vial with 300 mL work solution, containing 90% anaerobic sludge and 10% BA medium, with around 10 g/L (nonpretreated) glycerol.
Isolated colonies were then used to inoculate MOPS (morpholinepropanesulfonic acid) minimal media (TekNova, Hollister, CA) and incubated overnight with shaking (220 rpm) at 37°C, and then overnight cultures were used to inoculate batch cultures grown with continuous sparging aerobically (70% N2, 25% O2, and 5% CO2) or anaerobically (95% N2 and 5% CO2) as previously described [ 32].
These frozen stock cultures were used to inoculate precultures for all fed-batch experiments.
Cultures were used immediately.
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