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Samples from these synchronous batch cultures were removed at specific cell cycle time points for determination of cell concentrations, cell sizes, and analyses of DNA content and protein.
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Those containing nauplius batch cultures were inspected at least three times daily and dead larvae were removed.
As such, during the log phase growth of each batch, culture samples were removed and analyzed for the activities of PTA, PTB and AK in the irradiated and wild-type strains.
The batch cultures were exposed to continuous illumination between for 4 days.
Scale-up batch cultures were carried out in a 3.6 L bioreactor containing 2 L fermentation medium.
Batch cultures were carried out as follows.
Biomass and metabolite concentrations in batch and chemostat and batch cultures were determined as described by Guadalupe et al.[ 6].
However, for cumulative hydrogen volume, it is described here that at each observational period all the amount of gases produced including H2 within the headspace of the batch culture fermenter was removed for analysis.
In general, stationary phase batch cultures are more resistant to stress than exponential phase cultures.
This might be due to the fact that batch cultures are intrinsically never fully standardized.
Batch effects were removed by aligning the within-batch medians for all measurements.
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