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Exact(12)
Batch cultures were performed in 5 L fermenters (NBS Bioflo 115, USA) with 10% (v/v) inoculum size and 3 L working volume.
Batch cultures were performed using an organic culture broth volume ratio of 1 5 (v/v) for the whole set of the 50 mL cultures.
Thirteen rounds of repetitive batch cultures were performed for a total of 265 generations.
Four independent batch cultures were performed for each condition and each strain.
Batch cultures were performed in two independent biological replicates, whereas, for continuous cultures steady states were obtained in technical duplicates.
Submerged carbon-limited bioreactor batch cultures were performed and maintained starving up to six days after carbon depletion.
Similar(48)
Large volume fed-batch cultures were performed in a DasGip fermentor (SaniSure Inc, Moorepark, CA, USA) with 1 L working volume.
Fed-batch cultures were performed in a 5-L fermenter (Sartorius, Germany) with conditions set to optimize growth and (R -3-HB pR -3-HBon.
Finally, high cell density fed-batch cultures were performed with GALG20, this time using another ColE1-type plasmid, NTC7482-41H-HA, in pre-industrial facilities using glucose and glycerol.
Finally, three fed-batch cultures were performed under different conditions to see the metabolism distinction: FB-L with feed medium contained 120 mM glutamine and 60 mM asparagine; FB-H with feed medium contained 60 mM glutamine and 120 mM asparagine; and FB-N with feed medium contained no glutamine and asparagine.
All fed-batch cultures were performed in duplicate.
More suggestions(19)
batch cultures were removed
batch cultures were inoculated
batch studies were performed
batch cultures were cultivated
batch runs were performed
batch cultures were incubated
batch fermentations were performed
batch blasts were performed
batch experiments were performed
batch cultures were carried
batch cultures were harvested
batch cultures were diluted
batch cultures were maintained
batch cultures were adjusted
batch cultures were grown
batch cultures were used
batch cultures were investigated
batch cultures were started
batch cultures were developed
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