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Batch cultures were maintained in acid-washed 250 mL polycarbonate Erlenmeyer flasks containing 100 mL of sterile Modified High Salt Medium (MHSM-1; ionic composition is presented in Table 6[19]).
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The fed-batch cultures were maintained until the cell viability decreased to less than 50%, which occurred at day 16 post-inoculation.
Batch oxygen-limiting cultures were maintained in 15 mL glass tubes (Bellco Glass, Inc).
Algal stock cultures were maintained as batch cultures in 50-mL culture tissue flasks with modified Woods Hole Medium (MWC) at a continuous light intensity of 90 100 mmol m−2 s−1 and 17.5 °C.
Suspension cultures were maintained in the dark.
Control cultures were maintained in SF-MEM alone.
Neuron cultures were maintained as described above.
Feeder-free cultures were maintained on gelatinized tissue culture dishes.
Both cultures were maintained in 5%O2/CO2atmospherere.
Control parasite cultures were maintained at 37 °C.
Drosophila cultures were maintained on standard media.
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