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Cells grown in batch cultures were harvested at 'early' and 'full' nitrogen-limitation and were compared with non-limited cells.
The batch cultures were harvested every 2 weeks by centrifugation of the cultures at 1812 g, 20°C for 10 min, discarding the supernatant, and resuspending the cells with fresh autoclaved WC-medium.
At the end of cultivation (72 hours for the first batch and 50 hours for the second batch), cultures were harvested and used for HPLC analysis.
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In a study by DeRisi and colleagues, batch yeast cultures were harvested for cDNA array analysis at gradually decreasing glucose concentration [ 5].
Supernatants of the bacterial cultures were harvested.
The cultures were harvested for 7 h after induction.
At the end of the experiments, cultures were harvested.
Following a further 24 h incubation, cultures were harvested.
Cultures were harvested by filtration.
Cultures were harvested 15 17 days after initiation.
Cell cultures were harvested by trypsinization.
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