Sentence examples for batch cultures were grown from inspiring English sources
Diluted batch cultures were grown in sterile square 1 L Nalgene bottles as single filaments with gentle bubbling, sufficient for preventing aggregates formation without harming the integrity of the filaments.
Batch cultures were grown from colonies in 15 mL tubes in a shaker incubator and used to inoculate turbidostat cultures.
Anaerobic bioreactor batch cultures were grown essentially as described [ 6], but with 20 g l-1 galactose and a sparging gas consisting of 10%CO2and90%90% N2.
Batch cultures were grown in 20 ml tubes with 5 ml of LB medium on a gyratory shaker at 200 rpm, at 37°C.
Batch cultures were grown in 10 ml PYG medium (5 g of peptone, 10 g of yeast extract, 10 g of glucose, 5 g of tryptone, 40 mg of K2HPO4, 19.2 mg of MgSO4.7 (H2O), 8 mg of CaCl2, 40 mg of KH2PO4, 0.4 g of NaHCO3, 80 mg of NaCl and 1.1 mg of FeSO4.7 H2or or M9 (AMRESCO-J863) media.
The most common suggestion has been a two-stage process whereby batch cultures are grown aerobically in the first stage, followed by a second stage where the algae are deprived of sulfur and produce hydrogen in an anaerobic environment (Melis et al., 2000).
Fed-batch cultures were grown in 5 cm diameter Drechsel's bottles with a 250-ml working volume and bubbled using air enriched with 5%% CO2 (v/v); in this case, F/2 growth media was enriched with added nitrogen, phosphate and iron sources (0.75 g/L NaNO3, 0.05 g/L NaH2PO4 and 0.0063 g/L FeCl3·6 H2O final concentrations).
A 50 ml batch culture was grown to saturation and harvested by centrifugation at 9,000 rpm for 15 min at 4°C (Beckman JA 25.5 rotor).
Inoculum for batch and fed-batch cultures was grown in the lipid production medium with urea substituted for (NH4 2SO4 to provide buffering.
