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The batch cultures were exposed to continuous illumination between for 4 days.
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Some cultures were exposed to different treatments.
Scale-up batch cultures were carried out in a 3.6 L bioreactor containing 2 L fermentation medium.
Batch cultures were carried out as follows.
Biomass and metabolite concentrations in batch and chemostat and batch cultures were determined as described by Guadalupe et al.[ 6].
After adaptation to the new media, batch cultures were started and sampled daily as described below.
Four independent batch cultures were performed for each condition and each strain.
In general, stationary phase batch cultures are more resistant to stress than exponential phase cultures.
This might be due to the fact that batch cultures are intrinsically never fully standardized.
For each test, 80-100 moseparatedseparated into 4 batches were exposed to impregnated filter papers, while a batch of 20-25 moservedes served as control.
The results of fed-batch culture in shaking flasks of three fed-batch cultures were summarized.
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