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Batch cultures were diluted to OD600nm = 0.2 into YEPD medium the next day.
Batch cultures were diluted to OD600nm = 0.2 the next day and harvested once they reached an OD600nm = 1.0.
Batch cultures were diluted to OD600nm = 0.2 into YEPD medium the next day and harvested once they reached an OD600nm = 1.0.
Similar(57)
Before samples were collected for fatty acid and pigment analysis (see below), cultures were acclimated to the different temperatures and irradiance levels in semi-continuous batch mode for at least one week; fast growing cultures were diluted with fresh medium to avoid nutrient limitation.
The growth cultures were diluted to 5 × 105 CFU/mL.
Bacterial cultures were diluted to an OD600 of 0.1.
Cultures were diluted in sterile water prior to use.
Overnight cultures were diluted to an OD600 of 0.1 to make the starting cultures.
Overnight cultures were diluted 1 100 and plated on LB agar.
Cultures were diluted and plated onto solid YPD media.
The overnight cultures were diluted to 10 CFusing1 usterileerile broth.
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