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To investigate the hydrogen isotope systematics during methanogen growth, the initial δDH2O and δDH2 values in the batch cultures were adjusted by adding D2O and DH molecules (Table 2).
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Scale-up batch cultures were carried out in a 3.6 L bioreactor containing 2 L fermentation medium.
The batch cultures were exposed to continuous illumination between for 4 days.
Batch cultures were carried out as follows.
Biomass and metabolite concentrations in batch and chemostat and batch cultures were determined as described by Guadalupe et al.[ 6].
After adaptation to the new media, batch cultures were started and sampled daily as described below.
In general, stationary phase batch cultures are more resistant to stress than exponential phase cultures.
This might be due to the fact that batch cultures are intrinsically never fully standardized.
Experimental batch effects were adjusted for by including experimental batch as a covariate in the statistical model [ 56].
The results of fed-batch culture in shaking flasks of three fed-batch cultures were summarized.
Initial working volumes for the batch and fed-batch cultures were 4 and 3.5 L, respectively.
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