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Bacteria form biofilm not only in static batch cultures such as we used but also in batch cultures on orbital shaker [ 40], and in flow-through chemostats [ 41].
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Various factors can limit growth and/or production in a batch culture, such as nutrient and light availability and downstream effects thereof.
Furthermore, we used MFA method to study different processes of FB-L and FB-H during the stationary phase of fed-batch culture, such as glutamate metabolism and TCA cycle.
Batch culture methods, such as growth within microtitre plates, enable high throughput but do not allow for detailed microscopy of the biofilm (Merritt et al. 2005; Peeters et al. 2008).
Nerd culture, such as it is, is being HIJACKED~!
Ca. Pelagibacter ubique cells used in microarray experiments were grown in batch cultures as described above.
Biomass and metabolite concentrations in batch and chemostat and batch cultures were determined as described by Guadalupe et al.[ 6].
These have evolved since 1988 in Davis-Mingioli (DM) minimal media [ 12] batch cultures containing glucose as a growth substrate.
Measuring growth laws in dynamical situations - such as batch cultures which deplete substrate and enter the stationary phase [ 5] - is much more complicated.
Population size in batch cultures was measured both as the number of living cells and as total population biomass.
Batch cultures were carried out as follows.
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