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After glucose depletion, the batch cultivation was continued as a chemostat.
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Cultivation was continued further 3 h till the end of cultivation time.
Upon consumption of glucose, identified by a sharp drop of on-line monitored oxygen consumption, expression is induced and cultivation is continued under glucose-limited conditions as fed-batch process.
Batch cultivation was performed until all the xylose was depleted (around 24 h).
Once the concentration of dissolved oxygen increased rapidly, indicating that all the ethanol produced during batch cultivation had been depleted, batch cultivation was changed to fed-batch cultivation.
The working volume of batch cultivation was 500 mL.
Each autotrophic batch cultivation was carried out in duplicate.
Batch cultivation was changed to fed-batch cultivation when the concentration of dissolved oxygen increased rapidly, showing that all the sugar and the ethanol produced during batch cultivation had been depleted.
Aerobic batch cultivation was done in an incubator shaker at 37°C and 100 rpm.
Batch cultivation was performed at a stirrer speed of 700 rpm.
Batch cultivation was initiated with inoculation of 4.3 liter minimal medium with 109 conidia liter-1.
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