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Batch chromatography with a recycle stream is a popular and simple technique to separate a single target component in a complex mixture with moderate operating conditions.
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Emphasis is given to the potentialities of the process compared to single-column batch chromatography with recycling for the same amount of stationary phase.
First, the possibility of substituting the batch affinity chromatography with an FPLC-based alternative was evaluated.
As typical for separation processes, single unit batch chromatography exhibits a trade-off between purity and yield.
The aim of the present study was to investigate the usage of a two-column batch-to-batch recirculation process step to increase the performance compared to single-column batch chromatography at a high purity requirement.
The decisional tool was used to evaluate the economic and operational feasibility of whole mAb bioprocesses employing PCC affinity capture chromatography versus standard batch chromatography across a product's lifecycle from clinical to commercial manufacture.
It is shown that the bypass strategy enhances productivity of batch chromatography without an increase in the eluent consumption.
The performance of the MR-SSR process is compared to batch chromatography by means of a simulation study.
Proprietary simulation software was used to optimize SMCC and to evaluate the potential gains compared with batch chromatography.
Recombinant HP-NAP overexpressed in E. coli was subjected to DEAE Sephadex negative mode batch chromatography at pH 8.0 with the ratio of protein to resin being 1.5 mg/ml.
Therefore, DEAE Sephadex negative mode batch chromatography presented in this study is a simple and efficient method to purify recombinant HP-NAP expressed in E. coli.
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CEO of Professional Science Editing for Scientists @ prosciediting.com