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Pfam domain enrichment analyzes were carried out by performing a batch annotation of the P. anserina proteins at http://pfam.sanger.ac.uk/.sanger.ac.uk/
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Functional annotation chart was used to identify the significant batch annotation and GO terms that were most pertinent to the input data.
The batch annotation process is very easy and fast using NIST MS PepSearch.
Since many of these portals do not incorporate gene list upload features for batch annotation, users must, in certain cases, search individual gene candidates one at a time.
Functional annotation of dense PPI module is given by the Database for Annotation, Visualization and Integrated Discovery, i.e., DAVID http://david.abcc.ncifcrf.gov/, which accepts batch annotation and conducts gene set enrichment analysis.
Annotation of the non-coding genome.
Functional annotation of the proteins was performed using GO and KEGG annotation.
Annotation of the An.
Annotation of the Tr.
Gene ontology (GO) annotation was derived from batch queries of the Database for Annotation, Visualization, and Integrated Discovery (DAVID) Bioinformatics tool or manually through individual NCBI Entrez Gene queries [ 32, 33].
CellCognition [ 33] is a freely available software platform that includes high-throughput batch processing and annotation of complex cellular dynamics such as the progression of single cells through distinct cell division states.
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