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Copeptin levels were measured by batch analysis with a commercial chemiluminescence sandwich immunoassay (B.R.A.H.M.S LUMItest CT-proAVP, B.R.A.H.M.S AG, Hennigsdorf/Berlin, Germany), as described in detail elsewhere, with a lower detection limit of the assay of 0.4 pmol/L and the functional assay sensitivity (<20% interassay CV) of < 1 pmol/L) [29, 30].
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Batch analysis of over a thousand Alu sequences can be performed with this program.
As a quality control measure, all images were reviewed after batch analysis and cores with poor tissue segmentation were excluded.
DAPI intensity is not correlated with batch (analysis of variance [ANOVA]: algae P = 0.34 and bacteria P = 0.44).
We determined the linearity of calibration graphs with each batch analysis of the samples (approximately R2 > 0.999) and calculating ion balances (cation and anion).
They also developed a web-based plant small RNA meta-analysis toolbox, PsRobot, to meet the increasing need for batch analysis of small RNAs (sRNAs) in plants with the development of high-throughput sequencing technology [12].
In this article, we adapt the partially manual QuantMap method to a rapid automated system with an easy-to-use interface, opening up for moderately larger datasets as well as integration with other tools in batch analysis.
The Center has a 100-node computing cluster for batch analysis jobs.
For CNAG version 2.0, we set the reference pool to include all samples and performed an automatic batch pairwise analysis with sex-matched controls.
All batches that were positive in the pan-BTV-rRT-PCR analysis with a cycle threshold (Ct) value <37 (n = 464) were confirmed as BTV positive.
To minimize potential batch effects, analysis with dChip was performed in groups of approximately 50 probands/subjects according to when the arrays were hybridized.
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