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The most intense anti-mouse GAT4 immunoreactivity was localized to a few TRCs in the basolateral portion of the taste bud and in the cells surrounding the taste buds.
Due to these differences in terminology between rats and mice, our GAT4 findings parallel the report of GAT3 in rat taste cells [43], including the localization of the protein at the basolateral portion of the taste buds.
Thus, across mammalian species, these GAT transporters are localized primarily in the cells lining the basolateral portion of the taste bud, presumably where they function to remove GABA that has been released from a synapse.
Moreover, a GABA reuptake system which is critical to the physiological function of a GABAergic signaling pathway is expressed in the basolateral portion of the taste buds, the primary site of interaction between taste receptor cells and their post-synaptic targets.
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The signals were in the basolateral portions of the initial and transitional segments of anterior tubules (Figure 5B), which are the segments thought to function in excretion of divalent cations.
However, our mouse GAT4 immunolabeling was very consistent and was primarily restricted to the basolateral portion of a few taste cells and the cells immediately next to the taste buds with very little to no labeling in the apical portion of the taste bud.
Studies of liver tissue slices demonstrated that immunoreactivity for heparan sulfate proteoglycan (HSPG), but not other tested proteoglycans, was distributed along sinusoidal borders of liver; this immunoreactivity appeared associated with nonparenchymal cells of the sinusoids and with the basolateral portion of hepatocytes.
For recordings, pipettes from borosilicate glass capillaries were prepared using a horizontal puller (Sutter Instrument, Novato, CA, USA) backfilled with aCSF (resistance 2 4 MΩ) and placed at the upper portion of the basolateral nucleus22.
If a blastomere undergoes asymmetric division, each daughter cell inherits a distinct portion of the polarized membrane, either apical and basolateral or just basolateral, resulting in the differentiation of a TE cell and an ICM cell respectively [1].
We demonstrate here that hepcidin is enriched in the apical portion of the cholangiocytes which is compatible with a luminal secretion, while it is found at the basolateral membrane in hepatocytes, which are known to release hepcidin into the bloodstream [5], [19].
(See a portion of the strip below).
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