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We evaluated the transepithelial resistance developed between the apical and basolateral compartment of the NHBE cells in transwell inserts from day 1 to 21 of ALI culture from six independent NHBE cell cultures.
Cells were treated in the basolateral compartment of the bicameral system and exposed for 6 consecutive days to daily renewed 7.5 µM or 20 µM UCB, starting on day 4 after plating.
In validation experiments on sheets of terminal ileum, bilateral addition of forskolin plus IBMX elicited a robust GLP-1 secretory response into the basolateral compartment of the chambers.
Other secreted proteins such as e.g. metalloproteinases were detected comparably in the apical and basolateral compartment of the transwell cultures (data not shown) further endorsing the specificity of CTGF secretion.
Pathogens are then recognized on the basis of their invasiveness and sensed by pattern-recognition receptors (PRR) expressed either in an intracellular way or in the basolateral compartment of the epithelium [ 126].
However, no conjugates could be detected in either the apical or basolateral compartment of the transfected or parental (MDCK II cells, and so only efflux of quercetin aglycone was observed, which suggests that the cells did not contain UDP-glucuronosyl transferase nor aryl-sulfatase activity.
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Prior work in hair cells of different species has shown the presence of β-catenin and Na/K ATPase, two proteins identified as classically localized in the basolateral compartment of polarized epithelial cells, to be present in the basolateral surface of hair cells.
However, αB crystallin labeling was found only in the exosomes isolated from the basolateral compartment of severely stressed cells (Figure 5, panel c) and not in those isolated from unstressed cells (Figure 5, panel b).
The reaction mixture was added into the basolateral compartment of Caco2-BBE monolayers grown on filters for 10 min at 37°C.
We next evaluated the capacity of apically applied Bb to inhibit TNFα-induced CXCL8 production in the basolateral compartment of polarized HT29-19A epithelial cells.
However after 24 h of treatment, NPs were not found internalised in the basolateral compartment of endothelial cells.
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