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Four weeks later, the 50 L surface culture was harvested by using a number 2 centrifuge fitted with a basket filter.
The suspension was then crushed through a 40-μm basket filter, and unwanted red blood cells were lysed by using red blood cell lysis buffer containing 0.02 Tris HCl (pH 7.4) and 0.14 NH4Cl.
To keep the inside area around basket, filter, lid area clean (from coffee ground spatter) simply place a second filter over the top of the grounds in the first filter.
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Clean the basket and filter with a plastic — again, not metal — bristle brush.
Basket shaped filters are even easier, just cut out a circle of muslin as large as your paper filter smashed flat!
That includes ladders, baskets, hoses, filters, pumps, heaters, and any decorative pool fittings.
PORTAFILTER The filter basket and handle on an espresso machine.
Once ground, the coffee must be tamped with just the right amount of pressure into a filter basket.
A pod is a small sealed foil pouch that contains ground coffee that can be put directly in the filter basket for making espresso.
Remove and empty the filter basket at the base, if your model has one, because it can also fill up with debris.
If you look in the filter basket of the expensive batch brewer behind the counter of a serious coffee bar, you'll see a scalloped filter sitting on a flat bed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com