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Our results obtained with a livestock design and those of Schadt et al. obtained with mice indicate that the identification of subgroups in a population on the basis of transcriptome profiles would be an effective way to improve the power of QTL detection by linkage analysis.
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In addition, as previously reported by Schadt et al. [ 10] using a different animal design, we showed that the identification of animal subgroups on the basis of their transcriptome profiles is an effective way to partially eclipse the polygenic effects which interfere in classical QTL analyses.
In conclusion, the ESNATS assay battery allows classification of human DNT/RT toxicants on the basis of their transcriptome profiles.
Results of transcriptome profiling were validated using real-time PCR in 27 selected genes.
Li, S. et al. Multi-platform assessment of transcriptome profiling using RNA-seq in the ABRF next-generation sequencing study.
Sensitivity studies using mutants constructed on the basis of the transcriptome profiling confirmed a role for several such genes in antibiotic resistance, validating the usefulness of the approach.
Antibiotic susceptibility studies using mutants constructed on the basis of the transcriptome profiling confirmed a role for several genes in antibiotic resistance, validating the usefulness of the approach.
Battle, A. et al. Characterizing the genetic basis of transcriptome diversity through RNA-sequencing of 922 individuals.
Primers used in the real-time PCR assay were designed on the basis of transcriptome sequence.
In addition, genome sequencing of several adaptive mutants revealed the genetic basis for some of the observed transcriptome profiles.
In addition, co-expression analysis between miRNAs and their targets was performed on the basis of the miRNA transcriptome and transcriptome profiling of leaves and storage roots; the expression levels of 28 targets were negatively correlated with that of their corresponding miRNAs.
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