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On the basis of this alignment, we suggest domains for which structural information will be useful to allow homology modelling.
A total of 100 models were generated on the basis of this alignment and scored using the DOPE statistical potential.
On the basis of this alignment, Arg301, Ser295, Tyr139, and Trp134 were found to be highly conserved among these enzymes but not among members of the DHDH family.
On the basis of this alignment, and using Bioedit and Phylip package [ 49] a phylogenetic tree was reconstructed with the Maximun Parsimony method setting a bootstrap value of 2000 [ 50- 52].
Non-aligned N-ends were trimmed, leaving the master alignment presented in Figure 4 and Additional file 3. A consensus HyPRP pattern was developed on the basis of this alignment and verified by MyHits pattern search of the Arabidopsis GenBank proteins at the MyHits website [ 44, 45], where it retrieved the majority of the previously known HyPRP genes.
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Interestingly, the horse ortholog of FFA2 does not appear likely to share pharmacology with bFFA2 on the basis of this sequence alignment (Supplemental Fig. S1), this despite the fact that the horse is also heavily reliant on nondigestible carbohydrates, and, in fact, shows SCFAs concentrations in the digestive tract similar to those observed in ruminants (39).
This region was isolated by PCR with primers designed on the basis of the alignment of conserved sequences from the alkB gene region from different n-alkane degrading Rhodococcus bacteria.
The positions of SSR markers were determined on the basis of the alignment between the primers and the genomic sequence of Nipponbare; a maximum of one mismatch or indel was allowed in e-PCR.
We defined the alignment time of each step as 0 s, and calculated mean values of a 0.2 s time window on the basis of the alignment of each step.
On the basis of the alignment, structures were built using Model_Mult of MODELLER package.
The major loci were scanned on the basis of the alignment of 11 mitogenomes from four populations for proper molecular markers.
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