The set of the significantly differentially expressed genes was selected on the basis of their expression in adipose cells according to the IPA knowledge-base.
To verify the quantitative results of the RNA sequencing experiments, six genes were selected for RT-qPCR analysis on the basis of their expression levels in sequencing data and importance in the regulation of betalain biosynthesis.
These compartments of cells can be resolved on the basis of their expression of cell surface antigens.
These cells can be enriched from heterogeneous tumor cell populations on the basis of their expression of cell-surface markers.
CSCs have been isolated from colon cancer on the basis of their expression of the cell surface marker CD133 [12] [26], [26].
High-grade gliomas (grade III and IV) have been classified into three subclasses on basis of their expression profiles: pro-neural, proliferative and mesenchymal [24].
After depletion of B cells by magnetic bead cell sorting (Miltenyi Biotech), Tregs were removed on the basis of their expression of CD25 or CD62L.
Similar to effector T cell populations, Treg-cell populations are subdivided on the basis of their expression of homing receptors into subsets that are predicted to migrate preferentially to certain tissues [39].
Purified microglia were characterized by immunocytochemistry on the basis of their expression of the pan haematopoietic marker CD45 and of the monocyte/macrophage markers CD11b, F4/80 and CD68.
Although many of these studies have not detailed predictive sets of genes that could be used to make a diagnosis of CFS on the basis of their expression, we showed previously that the expression levels of 88 "CFS reporter genes", identified using microarrays, could assign individuals to CFS disease or healthy control groups following quantitative PCR of PBMC RNA [7], [8].
About a decade ago, the need for a rapid and reliable marker for the engraftment potential of haematopoietic stem and progenitor cell (HSC) transplants has led to the development of flow cytometric assays to quantitate such cells on the basis of their expression of CD34 [13].
