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Sequence (1.9×) of the domestic cat (Felis catus) [ 14] was assembled first by reconstituting cat contigs on the basis of their alignment with the dog assembly, then aligning these revised contigs with a cat radiation hybrid (RH) map.
The tools can be organized in various subgroups on the basis of their alignment strategies.
This allowed to compare sweet orange gene models (see Methods) and two custom genome annotation tracks, representing the 61 GST encoding transcripts we assembled, and 1,611 ESTs putatively encoding GSTs on the basis of their alignment to genomic sequences.
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We wondered whether we could superimpose the FATC domain of Tor1 on the structure of the PI3K catalytic subunit on the basis of their sequence alignment.
The size of rrnS and rrnL genes in C. sinicus were calculated to be 654 bp and 1,139 bp, respectively, on the basis of the alignment and comparison of their counterparts in N. cristatus.
Nucleotide sequences were then aligned, on the basis of protein alignment, with the Tranalign program of the EMBOSS package.
The rate of increase in reinforcement cannot be explained solely on the basis of GNS alignment.
On the basis of this alignment, we suggest domains for which structural information will be useful to allow homology modelling.
The positions of SSR markers were determined on the basis of the alignment between the primers and the genomic sequence of Nipponbare; a maximum of one mismatch or indel was allowed in e-PCR.
This region was isolated by PCR with primers designed on the basis of the alignment of conserved sequences from the alkB gene region from different n-alkane degrading Rhodococcus bacteria.
Three mutant SSUs were obtained from spontaneous ValR bacterial mutants and three more were designed on the basis of an alignment of SSU sequences from valine-sensitive and resistant isozymes, or consideration of the molecular model developed here.
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