Sentence examples for basis of the sequences obtained from inspiring English sources
5'-RACE PCR was performed according to instruction manual using gene specific primers designed on the basis of the sequences obtained by 3'-RACE.
A new 5' primer was designed on the basis of the sequences obtained (5'-ACCTGGAGTGCCGTAGGCTGTCCTTCTTG-3') and used to clone the remainder of the 5'end under the same conditions.
The present paper reports, on the basis of the sequences obtained, that modifications in these features can be related to the genetic origin of the pollen analyzed.
On the basis of the sequences obtained, we designed new specific primer pairs for the Bat-CoV/Philippines/Diliman1552G1/2008 sequence (5′-TGATTTCTGCAATGATACTTGGTTC-3′ and 5′-ACTTGATGATCTGTAACAACAATCG-3′) and for the Bat-CoV/Philippines/Diliman1525G2/2008 sequence (5′-TACAACCTACGCTGCAACTC-3′ and 5′-ATGAGTGTGCACAAGTGCTTAG-3′).
To compare the sequences obtained during this study with those of other rickettsiae, sequences present in GenBank were selected by means of BLAST as well as on the basis of previous reports (1, 12).
On the basis of the mitogenome sequences obtained, four primer combinations from relatively conserved regions were designed for screening polymorphism loci in the C. sinicus mitogenome.
On the basis of nucleotide sequences obtained, the pairs of species-specific primers were designed for each species studied.
Allele numbers for each locus were assigned on the basis of comparison of sequences obtained here to those deposited at the public MLST database (http://pubmlst.org/calbicans/).org/calbicans/
The miR-143-specific forward primer sequence was 5′-TGAGATGAAGCACTGTAGCTC-3′ and was designed on the basis of miRNA sequences obtained from the miRBase database.
Specific forward and reverse primers were designed by Primer3 software (http://bioinfo.ut.ee/primer3-0.4.0/ .ee/primer3-0.4.0/ene sequences onthened from the Genbasis
An aliquot of these reactions (2 μl) was then directly used as template in all PCR reactions in the presence of 100 pmol each of specific primers designed on the basis of sequences obtained from the Ah transcriptome [ 30] (see Additional file 12).
