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On the basis of the sequences of molecular markers Neubig et al. (2012) proposed another circumscription of the genus.
Primers were designed on the basis of the sequences available at the NCBI gene bank and produced by DNA technologies (Aarhus, Denmark).
5'-RACE PCR was performed according to instruction manual using gene specific primers designed on the basis of the sequences obtained by 3'-RACE.
A new 5' primer was designed on the basis of the sequences obtained (5'-ACCTGGAGTGCCGTAGGCTGTCCTTCTTG-3') and used to clone the remainder of the 5'end under the same conditions.
The Technical Appendix, shows the phylogenetic trees generated on the basis of the sequences of HA, NA, nucleocapsid protein (NP), and NS segments.
The present paper reports, on the basis of the sequences obtained, that modifications in these features can be related to the genetic origin of the pollen analyzed.
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The data are reconciled on the basis of the sequence structure of copolymer chains.
On the basis of the sequencing of a single Fuzhou-S individual (Supplementary Table 1, SI), SOAPsv80 was employed for annotating structural variations.
On the basis of the sequence of model selection, two hybrid models (ARIMA-MLP and MLP-ARIMA) are presented in the next section.
All primers of miRNA target genes were designed on the basis of the sequence of contigs.
Oligonucleotide probes were designed on the basis of the sequence of the centromeric and telomeric AP-1 sites of PIE.
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