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Sections were also scored semi-quantitatively on the basis of staining intensity as negative = 0; mild = 1; moderate = 2; intense = 3 [50].
Sections were also scored semi-quantitatively on the basis of staining intensity as negative = 0; mild = 1; moderate = 2; intense =3.
Some subunits may be differentiated on the basis of staining intensity or muscle fibre size, indicating that subunits may be composed of different fibre types [14], which is not unusual in invertebrates [18].
The immunolabeled cells inside the ROI were automatically counted on the basis of staining intensity.
Expression level was determined on the basis of staining intensity and percentage of immunoreactive cells.
Staining in each compartment was scored on a scale from 0 to 3 on the basis of staining intensity.
Similar(52)
Staining intensities were scored as the percentage of stained nuclei independent of staining intensity.
Comparisons of staining intensity are shown in q and r.
Comparisons of staining intensity are shown in j and k.
Comparisons of staining intensity are shown in i and j.
Quantifications of staining intensity were performed as previously described (46).
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