Sentence examples for basis of spot intensity from inspiring English sources

Arrays were quantified on the basis of spot intensity relative to background, adjusted for experimental variation between arrays using average intensity over multiple channels, and fitted to a previously described error model [29] to determine significance (type I error).

The oligonucleotides corresponding to each gene was spotted in triplicate, to increase precision of spot intensity measurements [59].

Spot detection, matching and quantification of spot intensity were performed using the DeCyder 2D Software, Version 7.0 (GE Healthcare, Freiburg, Germany).

was then used to process array images, align spots, integrate robot-spotting files with the microarray image, and to export reports of spot intensity data.

Two of these were also identified among the 19 most abundant proteins on the basis of protein spot intensity after proteome fractionation on 2D gels (Dedieu et al. 2013).

For detailed data of spot intensities see the online supplement.

However, substituting a single value for all missing spot intensities would skew the distribution of spot intensities considerably.

Random excision and subsequent MS of 270 spots over the range of spot intensities yielded 683 distinct proteins.

The PDQuest software compares images of 2-DE gels to determine differential protein expression and accurately identifies increased or decreased proteins on the basis of spots staining intensity.

This software allows the identification of differentially expressed proteins in the different groups, and accurately detects increased or decreased proteins on the basis of spots staining intensity.

The algorithm is based on the division of the spot intensity by the sum of all spot intensities on the respective gel.