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Cells were collected at medium flow rate and sorted on the basis of "single cell" and "purity".
However, there was no comprehensive data regarding the direct confirmation of quiescent characteristics of ASCs on the basis of single cell experiments in vivo and in vitro.
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On the basis of single-cell measurements and a dynamic growth model, we identify the major determinants of population growth and show that both physiological memory and environmental durations can strongly modulate the emergence of resistance.
The cells were resuspended in PBS and sorted on a fluorescence-activated cell sorter (FACS Aria SORP, BD Biosciences, Erembodegem, Belgium) on the basis of single-cell viability and the presence of GFP and DsRed.
Labeled cells were sorted using a fluorescence-activated cell sorter (FACS Aria, BD Biosciences) on the basis of single-cell viability and the presence of double-positive GFP and DsRed fluorescence.
The hypothesis that the LDAP component observed in the present study may reflect response preparation processes in the PRR that are analogous to those identified on the basis of single-cell recordings is of course largely speculative at the present moment.
Originally formulated on the basis of single-cell recordings in monkeys (Tian et al. 2001) and anatomical tract tracing (Romanski et al. 1999), evidence from human brain imaging studies has accumulated in support of separate processing streams for spatial versus nonspatial auditory information (Arnott et al. 2004).
Quantification of single cell responses.
To determine whether the enhanced pluripotency of mES cells cultured in 2i has a transcriptional basis at the level of single cells, expression of several key markers of pluripotency and lineage commitment was measured directly in individual cells by qPCR (Fig. 1A).
An example of such a technology in development is single-cell genomics, which allows for detailed genomic and transcriptomic studies on the basis of a single cell [ 1, 2].
Here, we discuss the need for single cell studies, referring to recent studies of single cells using high-throughput techniques, and their utility for understanding the transcriptional basis of cell fate choices.
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