Sentence examples for basis of sequence specificity from inspiring English sources
To characterize the structural basis of sequence specificity and flexibility of the Escherichia coli Hsp70 chaperone DnaK, we have analyzed crystal structures of the substrate binding domain of the protein in complex with artificially designed peptides as well as small proline-rich antimicrobial peptides.
Analysis of these comprehensive specificity profiles is facilitated through the use of sequence-specificity landscapes (SSLs).
Currently, it is not possible to predict in detail the specificity of TEIIs on the basis of sequence analysis, and they must be studied experimentally case by case.
However, the specificity of a recombinant protein cannot be reliably predicted on the basis of sequence homology alone.
Generally, the specificity of the CoA ligases for 4-hydroxybenzoate and benzoate is difficult to predict solely on the basis of sequence similarity47,48.
Our results show that the full DNA-binding specificity of uncharacterized TFs to individual k-mers can be predicted on the basis of similarity in protein sequence alone, given the sequence specificity of closely related members of the same TF family, and (preferably) knowledge of the DNA-contacting residues.
For each gene, two cDNA probes (450 550 bp) were designed from different regions (coding region and 3′-untranslated region [3′-UTR], respectively) on the basis of simple criteria concerning length and sequence specificity and spotted on the macroarray.
Understanding the sequence and structural basis of interaction specificity in this family is important, as it may allow the prediction of new Bcl-2 family associations and/or the design of new classes of selective inhibitors to serve as reagents or therapeutics.
One potential mechanism for ensuring pairing-interaction specificity is the use of sequence-specific DNA-binding proteins that can bind simultaneously with two or more recognition sequences.
Wu RA, Collins K. Sequence specificity of human telomerase.
