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The primers were designed on the basis of published sequences Acc #.
Primers were designed on the basis of published sequences from domestic porcine (Sus scrofa).
Primers (Forward: 5'-CGGGATCCATGGAGGACG-3', Reverse: 5'-GGAATTCCACTGAGATCACT-3') used for isolating putative DUB genes are made on the basis of published sequences [GenBank: XM_252830].
Design of synthetic oligonucleotide primers and fluorescent probes for each gene investigated was performed on the basis of published sequences (Table 1).
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Primer 'F' was published by Sperling et al. [23] as part of the Yponomeuta malinellus sequence; the other three primers were used by Brown et al. [30] and designed by members of the Rick Harrison laboratory at Cornell University on the basis of comparisons of published sequences from D. yakuba [29] and Apis mellifera [31] as entered in GenBank [32].
Numbers in parentheses are NCBI accession numbers of published sequences.
On the basis of partial published sequences, a set of primers was designed for amplification of the F gene of HMPV (5, 7).
On the basis of the published sequence of the porcine MyoG gene, ten pairs of primers were designed, and one polymorphism was found in the PCR product amplified with In2-3 primers.
The oligonucleotides designed using Primer Premier 5.0 on the basis of the published sequence of chitinase (ChiB) gene [ 28] were synthesized and used for determination of the chitinase gene sequence.
All oligonucleotide primer sets were designed on the basis of published mRNA sequences.
We found eight distinct full-length (659 to 667 amino acid) sequences which we divided into three groups on the basis of similarity to published sequences.
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