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On the basis of primer specificity and amplicon size, we determined host and parasite species (Technical Appendix Figure 1).
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Hence, the necessity of primers specificity.
β-Glucosidases may be classified into three groups on the basis of substrate specificity.
The genetic basis of host specificity for animal and plant pathogenic bacteria remains poorly understood.
On the basis of substrate specificity, they are classified as serine/threonine, tyrosine, dual specificity, or histidine protein phosphatases.
When designing primers, primer specificity and absence of primer-dimers were evaluated.
20 Sequencing of cloned RT-PCR products confirmed primer specificity.
Primer specificity and the formation of primer dimers were monitored by dissociation curves.
Primer specificity was verified via cloning of PCR product.
AAP primer specificity was assayed through use of control PCRs using untailed cDNA as template.
To guarantee specificity of AS primers, the proposed system incorporates a primer specificity enhancement technique widely used in experiment protocol.
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