Sentence examples for basis of obtained sequences from inspiring English sources

We then developed a set of nested PCR primers on the basis of obtained sequences to detect viral RNA (Technical Appendix Table 1).

The alignment of the obtained sequences was the basis for the design of 11 species-specific reverse primers located in the 16S rRNA gene region.

The alignment of the obtained sequences was the basis for the specific design of species-specific reverse primers (ITSArSil-R, ITSDir-R, and ITSMa-R) located in the ITS-1 region.

On the basis of obtained and published nucleocapsid sequences (2, 4 ), we developed a TaqMan reverse transcription PCR to detect viral RNA using the following primers and degenerate probe: forward, 5′-TTGGAAAGAATGGTGCTAAAACTG-3′; reverse, 5′-CATTAGGCACGTTACCATCAAATT-3′; and probe, 5′-TAGGAACRCGTGGCACCAACCAA-3′.

solely on the basis of obtained sensor readings.

All gaps were filled by designing new primers on the basis of sequences obtained from PCR products (Additional file 1).

Gene-specific primers were designed on the basis of sequences obtained for selected Tribolium genes and the 18S rRNA gene served as the endogenous control (normalizer).

An aliquot of these reactions (2 μl) was then directly used as template in all PCR reactions in the presence of 100 pmol each of specific primers designed on the basis of sequences obtained from the Ah transcriptome [ 30] (see Additional file 12).

A total of 28 gene-specific primer pairs (see Additional file 2 for primer information) were designed on the basis of sequences obtained for selected T. ni genes known to be involved in immune response in other insect species or were based on proteins identified as immune-responsive in our protein expression experiments.

Although on the basis of sequences obtained from their amplicons, TTSS2 genes in PMA339 seem to have independently evolved from the pandemic strain over a considerable time, they are still more closely related to the TTSS2 of V. parahaemolyticus than to those recently found in non-O1 or non-O139 V. cholerae (14 ).

Gene-specific primers were designed on the basis of sequence obtained for selected T. ni genes and several additional genes as potential housekeeping genes to serve as the endogenous control (normalizer).