On the basis of multilocus sequence analysis of chromosomal housekeeping genes, this group was found to be genetically distinct from other B. garinii strains.
This strain has been recently suggested for classification with 3 others as a subspecies within the species R. conorii on the basis of multilocus sequence typing (4 ).
At >62.50% similarity, 3 main clusters were found that matched with existing species on the basis of multilocus sequence analysis (ITS, CDC42, BT2, and ACT1), i.e., F. pedrosoi, F. monophora, and F. nubica.
An onsite investigation of the potential exposure site found the vector, Ornithodoros hermsi ticks, and 1 chipmunk infected with spirochetes, which on the basis of multilocus sequence typing were identical to the spirochete isolated from the patient.
On the basis of multilocus sequence data and allelic profiles for 53 specimens, analysis showed strong LD among 25 polymorphic loci, suggesting an overall nonpanmictic population structure of C. hominis.
This subpopulation was initially designated lineage C1 (Homan et al. 2002) and later renamed to clonal complex 17 (CC17) on the basis of multilocus sequence typing (MLST) data analysis (Willems et al. 2005, 2011).
LA-MRSA has now been identified largely as a single clonal complex on the basis of multilocus sequence typing (ST398), and this clonal complex has a demonstrated association with pigs, cows, and other animals (11, 12 ), although other clonal complexes have also been shown to be associated with livestock as well.
New Aspergillus species have recently been described with the use of multilocus sequencing in refractory cases of invasive aspergillosis.
All the strains had previously been characterized by means of multilocus sequence typing (MLST).
Bougnoux, M.-E., Morand, S. & d'Enfert, C. Usefulness of multilocus sequence typing for characterization of clinical isolates of Candida albicans.
