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We also investigate the molecular basis of melanic phenotypes from geographically distant populations of P. maniculatus and find that melanism has independently arisen at least three times and by different mutations in the same gene, Agouti, in two of those cases.
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Second, to study the genetic basis of other melanic phenotypes, we obtained tissue samples of melanic mice from natural history collections originally captured in two additional populations in Alaska (P. m. keeni) and Michigan (P. m. gracilis).
While the fitness consequences of the melanic phenotypes in this study are unknown, studies of pigmentation may be uniquely positioned to identify convergence and to uncover its molecular basis because pigmentation traits are easily recognizable and many of the genes involved in producing pigments are well characterized.
The large number of recruitment centres, despite the relatively high frequency of these tumours, was to ensure a wide range of different melanic phenotypes, by sampling various populations.
Researchers interested in the evolution of melanism have asked whether the melanic phenotypes exhibited by different taxa are produced via the same or different molecular mechanism.
The inheritance of the melanic phenotype in the New Hampshire strain of P. maniculatus was previously investigated by Horner et al. [17].
Though the small number of animals sampled does not allow us to rule out the involvement of other loci, these data strongly suggest that the aQ65term allele is the cause of the melanic phenotype in the Alaskan population.
Studies on the molecular basis of melanism in vertebrates have found that different genetic mechanisms can produce melanic phenotypes in different taxa [3].
Although we cannot rule out contributions of linked variation to the melanic phenotypes possessed by mice from New Hampshire and Alaska, given the likely effects of the Δ125kb and Q65term mutations and the known effects of null Agouti alleles in other taxa, it is very likely that these mutations represent the causative variation underlying these melanic phenotypes.
Ethiopian populations of D. melanogaster display uniquely melanic phenotypes not observed in other worldwide populations.
Chong, J. X. et al. The genetic basis of mendelian phenotypes: discoveries, challenges, and opportunities.
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