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In Affymetrix technology, the final expression level is computed on the basis of intensity levels of several independent probes matching the same target messenger RNA.
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Sites (n=9) were purposely selected on the basis of the intensity level of MDT (higher or lower).
CB1IR was scored on the basis of intensity (0 = absent up to 3 = high intensity) × distribution, giving a range of 0 3.
VEGF staining was scored on the basis of intensity and breadth of staining of the urothelium.
Therefore, the identification of TUs on the basis of uniform levels of signal intensities, using either RNA-seq or microarray analysis, is ambiguous and classified as weak evidence with two exceptions.
Expression level was determined on the basis of staining intensity and percentage of immunoreactive cells.
We are now in the final week of our Kickstarter project, and the intensity level is off the charts.
A standard curve could be generated on the basis of fluorescent intensity and protein concentration.
Each case was scored on the basis of the intensity and area of staining.
The immunolabeled cells inside the ROI were automatically counted on the basis of staining intensity.
Using Imaris software (Imaris×64 6.4.2), individual peroxisomes were identified on the basis of fluorescence intensity and diameter.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com