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Genes were filtered on the basis of fold change ≥2 and p value ≤0.05.
Most studies define up- or down-regulated genes on the basis of fold change in expression between two conditions.
Differentially regulated genes were defined on the basis of fold change, and corresponded to the uppermost 18.75th percentile of data sets formed using genes with normalized, expression ratios (infected/control) >1.01 or <0.99 in at least ten out of fourteen arrays.
The 152 transcripts with varying EC specificity are ranked on the basis of fold change in the primary set and fold change from the external datasets (e.g. REFEXA).
The patterns can be roughly separated on the basis of fold change: expression in ovule primordia regions results in smaller changes (approximately -1.4) and ovule and integument expression results in slightly higher fold changes.
For pathways analysis of more restricted gene sets consistent through bidirectional modulation of miRNA, we reduced the threshold further to include genes on the basis of fold change alone.
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As observed with the Otx2+/Oct4− group, further partitioning of the Otx2+/Oct4+ up) subcategory on the basis of fold changes in H3K27Ac levels, indicating that there are large subset of enhancers which show big increases in H3K27Ac, consistent with their activation and another subset that changes minimally.
This approach has the major advantage of skipping the identification of differentially expressed genes on the basis of fold changes and/or t-test or U-test analysis, a process highly sensitive to heterogeneity in the patient data and therefore often incapable of identifying relevant disease-specific genes.
Differentially expressed genes were detected on the basis of fold-change threshold (1.2-fold) and P-value (<0.05).
Nine target genes were selected on the basis of microarray results and reflected the whole range of fold change (both up-regulated and down-regulated genes in different samples).
Gene symbol, fold change, direction of fold change, and presence of CRE sites (CRE) are indicated.
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