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Another formulation on the basis of fluorescence experiments has been set up considering each chain to be labelled either with a Förster donor or a Förster acceptor.
In summary, we are able to demonstrate that the structural destabilization in the N-terminal segment of the T domain likely unpacks the overall folded structure, leading to separation between the N-terminal and C-terminal segments at pH 6.0, as previously suggested on the basis of fluorescence experiments and MD simulations.
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A series of fluorescence experiments based on tryptophan peptide fluorescence or with fluorescence labeled SUVs, were done to cover different aspects of peptide interaction with bilayers.
Open image in new window Fig. 1 Schematic illustration of the detection of Fe3+ on the basis of fluorescence enhancement and subsequently fluorescence quenching.
The basis of fluorescence methods lies in the ability of chlorophyll located in photosynthesis membranes to serve as a natural detector for the algae cells, emitting quanta fluorescence.
On the basis of fluorescence readings, 115.9 nmol of fluorophore-conjugated Lymphoseek 2 was isolated.
A similar conclusion was reached on the basis of fluorescence studies of translocation.
Using Imaris software (Imaris×64 6.4.2), individual peroxisomes were identified on the basis of fluorescence intensity and diameter.
Aniline derivatives cannot be detected on the basis of fluorescence, but only by UV detection.
Comparisons were made on the basis of fluorescence per μg protein.
On the basis of fluorescence amplitude, a simple threshold assigns each droplet as positive or negative.
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