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The clustering of genes on the basis of expression level is represented in a heat map (Fig. 1b).
On the basis of expression level and product profile, it was concluded that UGT1A3 was the isoform responsible for the formation of 8- O-monoglucuronsyl-B[ a]P-7,8-dione.
On the basis of expression level and relative activity, MAG lipase, ABHD12, and ABHD6 are estimated to account for 85%, 9%, and 4% of 2-AG hydrolysis in mouse brain, respectively.
Our designation of the two periostin loci in the fish species as "locus 1" and "locus 2" (see Additional file 1, Table S1 for genomic coordinates) was done on the basis of expression level as judged by the amount of transcript evidence mapped to either locus in the UCSC genome browser.
The genes analyzed above were divided on the basis of expression level, rather than the size of tissue/organ; genes in the top 30% quantile were considered to be highly expressed and those in the bottom 30% quantile were considered to be weakly expressed genes.
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The candidate miRNAs were further filtered on the basis of expression levels.
The two strategies were compared on the basis of expression levels, purification performance, enzymatic activity, bioreactor cultivations, and transcriptional profiling.
We first contrast the risk profiles of the cases and controls in subtypes created on the basis of expression levels of each of the genes in turn.
miRNAs selected for investigation in our study were further filtered on the basis of expression levels and previously published data [ 31].
The estrogen receptor gene (ESR1) appears among the top ranked genes in both analyses, confirming the extensive evidence in the literature that breast cancers classified on the basis of expression levels of this gene have distinctive etiologies.
Although this cannot be explained on the basis of expression levels it may be a consequence of the differential intracellular localizations of each of the proteins determined by elements in their variable N-terminal domains including motifs for myristoylation [ 24] and palmitoylation [ 45].
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