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All culture assays were normalized on the basis of cell viability by using the CellTiter-Glo assay from Promega.
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After chemical exposure, cell viability was analyzed by fluorescence detection of cell viability assays on a per microwell basis using a standard microarray scanner.
The data are presented as percentage of cell viability relative to unstimulated cells (0 μM).
Sensitive detection of cell necrosis is crucial for the determination of cell viability.
Cellular ATP content was determined as a marker of cell viability using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Sweden).
The results of cell viability are shown in Fig. 7.
Figure 5 Direct contact measurements and calculations of cell viability.
Hence 77% of cell viability could be observed (Fig. 3b).
IC50 value (concentration of compounds inhibited 50% of cell viability) was determined from the graph of cell viability vs absorbance.
Cell viability data were expressed as percentage of cell viability calculated.
Determination of cell viability.
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