Our findings build the direct link between the MT-based KCBP motor and the actin cytoskeleton, unravel the cellular basis controlling trichome cell shaping, and provide significant insights into the mechanisms of cytoskeletal regulation of cell shape determination.
In this study, our findings provide conceptual advances both in understanding the role of KCBP in regulating the interaction between cortical MTs and F-actin during trichome morphogenesis and in elucidating the cellular basis of trichome cell shape determination.
In order to decipher the genetic basis of plant cell shape patterning and growth, we employed, in this study, a reverse genetic approach by screening the loss of gene expressions in Arabidopsis T-DNA knockout mutants to gain insights into the mechanisms controlling cell morphogenesis in plants.
Bacteria display a wide variety of cell shapes.
Here, we quantitatively account for this wing-blade shape change on the basis of cell divisions, cell rearrangements and cell shape changes.
The main spindle region was defined on the basis of the cell body shape and the centrosomal region (both revealed by α-tubulin immunofluorescence) and the apical- and basal-most (peri- centromeric foci (DAperi- centromeric focire 2 A ).
Traditional simulations of these cell shape changes require that grids and boundaries be moved as the cell's shape evolves.
Potential targets were evaluated on the basis of shape to differentiate cell bodies from auto-fluorescent debris, and to reject the fluorescence from dendrites and neuropil whose somata were detectable in adjacent focal planes (Wang et al., 2013).
Small healthy follicles were classified as either columnar (n = 5) or rounded (n = 5) on the basis of the shape of the basally-situated granulosa cells [ 15, 16] as described in the Methods.
On the basis of spike shape, firing rate, and firing location, complex-spike cells with 1 or 2 firing fields were separated.
