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The limited proliferation potential of primary MECs causes significant technical problems for dissecting the molecular basis of cell cycle control in these cells.
To evaluate the distribution of treated cells on the basis of cell cycle properties, treated cells were washed twice with ice-cold PBS, collected by centrifugation and fixed in 70% (v/v) ethanol at 4°C for 2 h.
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Ratios of cells in the G0/G1, S, and G2/M phases of cell cycle were determined on the basis of their DNA content and presented as cell percentage at the end stage of the cell cycle.
To evaluate the molecular basis underlying the cell cycle arrest, we investigated the expression of cell cycle regulatory proteins.
Taken together, the activation of cell cycle and inhibition of apoptotic cell death may form the basis for the cancer cell survival in resistant RB.
Up- and downregulation of cell cycle and apoptosis regulator genes appeared to be the molecular basis for the former (Fig. 7).
While these studies revealed the role of cell cycle status in the engraftment of CD34+ cells during ontogeny, the molecular basis behind these observations remains unknown.
The distribution of cell cycle phases with different DNA contents was analyzed using HCS on the basis of acquiring 10,000 events for each sample.
Principles of Cell Cycle Control. 3 Units.
Uroz, M. et al. Regulation of cell cycle progression by cell cell and cell matrix forces.
(d), FACS analysis of cell cycle phases in synchronized PSFs.
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