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Ten amino acids that have high affinity to three different regions of t-PA are identified by the amino acids location method on the basis of candidate pocket structure of t-PA.
On maturation, MPE-primary cultures were sorted on the basis of candidate CSC-markers.
However, to answer this important question, the MPE-model is an appropriate, clinically relevant prototype to determine if cells that are segregated on the basis of candidate CSC markers are predictive of a distinct tumorigenic or invasive phenotype.
For example, we can now propose to test whether MPE-tumors that are segregated on the basis of candidate CSC-markers will display differences in in vitro surrogate measures of the CSC-phenotype (soft-agar colony formation, drug resistance, and/or matrigel invasion) from isogenic tumor cells that don't express candidate CSC markers.
On basis of candidate selection signatures identified by combining strategy, the genes close to the putative outliers were explored using the available annotation of the chicken genome (Annotation Release 102).
On the basis of candidate mRNA sequences, 11 pairs of primers were designed to amplify about 1 kb genomic fragments from the 20 genotypes for each of the 11 genes (Table 2).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com