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Specifically, Figure 4 of this paper was constructed on the basis of an alignment with the high-resolution structures of LUSH alcohol complexes.
Three mutant SSUs were obtained from spontaneous ValR bacterial mutants and three more were designed on the basis of an alignment of SSU sequences from valine-sensitive and resistant isozymes, or consideration of the molecular model developed here.
For instance, on the basis of an alignment of 344 unique sequences, Fu and Chang identified a highly conserved P-G doublet in non-animal sHSPs [36].
Phylogenetic analysis was performed on the basis of an alignment of protein sequences obtained using MUSCLE [ 53].
A phylogenetic tree generated on the basis of an alignment of the fiber knob domains of macaque and human adenoviruses is shown in Figure 4.
However, this may not be significant, given that the domain was defined on the basis of an alignment that included both archaeal and bacterial sequences.
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Thus, we focused on the N-terminus of PfRH5 and chose a 143-aa sequence of PfRH5 from Asn-31 to Val-174 on the basis of a Clustal alignment of PfRH5 with the phylogenetically close PvRBP1 [20] (∼22% overall similarity, data not shown).
Often, the exact phase of an indel cannot be reconstructed unambiguously on the sole basis of a multiple alignment.
The prediction of the mature form was on the basis of a sequence alignment with mature cpCKII from Sinapis alba as described in [ 17].
Previously, information on the likely structure of HicA toxins has been derived from the reported structure of TTHA1913, a protein of unknown function from Thermus thermophilus, which on the basis of a sequence alignment belongs to the HicA family [ 49].
The last column of Table 4 suggests how MTUHA indicators could be used in the hospital development plan and, as such, could form the basis of a similar alignment process for other areas.
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